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Genetics

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JoVE Journal Genetics
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
 

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Article DOI: 10.3791/59235-v • 08:54 min • March 29th, 2019
March 29th, 2019 •
1, 2, 1, 1, 1, 1
1Center for Epigenetics, Van Andel Research Institute, 2Amgen

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This protocol outlines the steps needed to generate a model system in which the transcription of an endogenous gene of interest can be conditionally controlled in live animals or cells using enhanced lac repressor and/or tet activator systems.

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In Vivo Application REMOTE-control System Endogenous Gene Expression Versatility Potency Robust Control Existing Methods Gene Function Expression Levels Spatio-temporal Manner Reversibility Of Phenotype Disease-related Genes Repression Target Gene Intron Repron R Lac Operators LacIGY Repressor Tissue-specific Promoter Repression Reversal IPTG Upregulation Target Gene Promoter Tat Operators RtTA-M2 Activators Doxycycline Concentration Adjustment Combined Systems
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