Journal
/
Génétique
/
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
Journal JoVE
Génétique
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Génétique
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

12,991 Views

•

10:09 min

•

June 27, 2017

DOI:

10.3791/55187-v

DOI:
10.3791/55187-v

•

10:09 min
June 27, 2017

•

18 Views
, , ,
1Department of Chemistry and Biomolecular Sciences,University of Ottawa, 2School of Biosciences,The University of Nottingham Malaysia Campus

Summary

Automatically generated

This study describes methods for the T7-mediated co-expression of multiple genes from a single plasmid in Escherichia coli using the pMGX plasmid system.

Explore More Videos

Inducible T7 RNA PolymeraseMultigene Expression SystemPMGXSynthetic BiologyBiochimieProtein ComplexesCo-expressionEscherichia ColiGene CloningRestriction Enzyme DigestionGel ElectrophoresisGel ExtractionLigationBacterial TransformationAntibiotic Selection

Read Article

Cite this Article

Hassan, M. I., McSorley, F. R., Hotta, K., Boddy, C. N. Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX. J. Vis. Exp. (124), e55187, doi:10.3791/55187 (2017).

Download .ris file

Copy

Share Video

.

Copy