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In vivo anvendelse af sen-kontrol-System for Manipulation af endogene genekspression
Journal JoVE
Génétique
This content is Free Access.
Journal JoVE Génétique
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

In vivo anvendelse af sen-kontrol-System for Manipulation af endogene genekspression

DOI:

08:54 min

March 29, 2019

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Chapitres

  • 00:04Titre
  • 00:40Overview of the REMOTE-control System
  • 01:50Modify the Gene of Interest for Repression by REMOTE-control
  • 06:04Manipulate Gene Expression In Vivo
  • 06:50Results: In vivo Repression and Activation Capability of the REMOTE-control System
  • 08:23Conclusion

Summary

Traduction automatique

Denne protokol beskriver de nødvendige skridt for at generere en modelsystem hvor transkription af en endogen gen af interesse betinget kan styres i levende dyr eller celler ved hjælp af forbedrede lac repressor og/eller tet aktivator systemer.

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