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Immunology and Infection

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JoVE Journal Immunology and Infection
Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy
 

Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy

Article DOI: 10.3791/58355-v • 08:01 min • August 17th, 2018
August 17th, 2018 •
*1,2, *1,2, 1, 1,3, 1
1Faculty of Biomedical Sciences, Institute for Research in Biomedicine, Università della Svizzera italiana (USI), 2Graduate School of Cellular and Molecular Sciences, Faculty of Medicine, University of Bern, 3Institute of Computational Science, Università della Svizzera italiana (USI)
* These authors contributed equally

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résumé
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In this study, we present a protocol to perform two-photon intravital imaging and cell interaction analysis in the murine tracheal mucosa after infection with influenza virus. This protocol will be relevant for researchers studying immune cell dynamics during respiratory infections.

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Imaging Cell Interaction Tracheal Mucosa Influenza Virus Infection Two-photon Intravital Microscopy Immune Responses Dynamics Respiratory Infections Immune Cells Tracheal Epithelium Surgery Neutrophil Interactions Lytic Cell Interactions Immune Cells Airborne Pathogens Image Analysis Heart Defects Intranasal Influenza Infection CD11c-YFP Mouse Supine Position Deep Sedation PR8 Inoculate Nasal Cavity Virus Delivery
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