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Cell Membrane Repair Assay Using a Two-photon Laser Microscope
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Immunologie et infection
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Journal JoVE Immunologie et infection
Cell Membrane Repair Assay Using a Two-photon Laser Microscope

Cell Membrane Repair Assay Using a Two-photon Laser Microscope

DOI:
10.3791/56999-v

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06:35 min

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January 02, 2018

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, , ,
1Department of Medical Genetics,University of Alberta Faculty of Medicine and Dentistry, 2Center for iPS Cell Research and Application,Kyoto University

Chapitres

  • 00:05Titre
  • 00:38Full-length Dysferlin (DYSF) Plasmid Cell Transfection
  • 03:09Two-photon Laser Wounding Assay
  • 05:19Results: Representative Fluorescence Quantification Over Time
  • 06:00Conclusion

Summary

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Traduction automatique

Cell membrane wounding via two-photon laser is a widely used method for assessing membrane resealing ability and can be applied to multiple cell types. Here, we describe a protocol for in vitro live-imaging of membrane resealing in dysferlinopathy patient cells following two-photon laser ablation.

Tags

Keywords: Cell Membrane RepairMuscular DystrophyTwo-photon Laser MicroscopeFibroblast CellsPlasma MembraneMembrane ResealingDysferlinTransfectionFM4-64 DyeTwo-photon Wounding Assay

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