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Immunology and Infection

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JoVE Journal Immunology and Infection
Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver
 

Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver

Article DOI: 10.3791/57191-v • 07:06 min • February 6th, 2018
February 6th, 2018 •
*1,2, *1,2, 3, 1,2
1Department of Anatomy, Yonsei University College of Medicine, 2Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, 3Department of Surgery, Yonsei University College of Medicine
* These authors contributed equally

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résumé
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We established a novel surgical protocol for two-photon imaging of live mice liver with minimal invasion. With this technique, we identified the detailed structure of the liver during lipopolysaccharide-induced endotoxemia. We anticipate that this method may be utilized to determine the effectiveness of various reagents treatment to hepatic leukocyte migration.

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