JoVE Journal
Biochemistry
Biochemistry
Ce contenu est en libre accès.
Chapitres
résumé
This is a protocol to study intracellular protein-protein interactions based on the biotin-avidin pull-down system with the novelty of combining cell-penetrating sequences. The main advantage is that the target sequence is incubated with living cells instead of cell lysates and therefore the interactions will occur within the cellular context.