Journal
/
Biochimie
/
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
Journal JoVE
Biochimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biochimie
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

8,594 Views

•

08:53 min

•

January 11, 2017

DOI:

10.3791/54971-v

DOI:
10.3791/54971-v

•

08:53 min
January 11, 2017

•

6 Views
,
1Department of Pharmacology, Center for Mitochondrial Diseases, The Cleveland Center for Membrane and Structural Biology,Case Western Reserve University School of Medicine

Summary

Automatically generated

This paper describes a method for assessing the interactions and assemblies of integral membrane proteins in vitro with various partner factors in a lipid-proximal environment.

Explore More Videos

Scaffold LiposomesLipid-proximal Protein-protein InteractionsMitochondrial DynamicsProtein ComplexesMembrane CompartmentsLipid TemplatesNative ConfirmationLipid CofactorsMitochondrial Fission MachineryHis-tagged ProteinsMffBuffer ABMELipid Extrusion

Read Article

Cite this Article

Clinton, R. W., Mears, J. A. Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro. J. Vis. Exp. (119), e54971, doi:10.3791/54971 (2017).

Download .ris file

Copy

Share Video

.

Copy