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Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
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October 21, 2016
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Summary
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Protein interactions are at the heart of a cell's function. Calorimetric and spectroscopic techniques are commonly used to characterize them. Here we describe fluorescence anisotropy as a tool to study the interaction between the protein mutated in the Shwachman-Diamond Syndrome (SBDS) and the Elongation factor-like 1 GTPase (EFL1).
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Gijsbers, A., Nishigaki, T., Sánchez-Puig, N. Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions. J. Vis. Exp. (116), e54640, doi:10.3791/54640 (2016).
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