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10:19 min
June 15, 2017
DOI:
10.3791/55765-v
小鼠卵母细胞的显微注射通常用于经典转基因( 即转基因的随机整合)和CRISPR介导的基因靶向。该协议审查显微注射的最新发展,特别强调质量控制和基因分型策略。
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Delerue, F., Ittner, L. M. Generation of Genetically Modified Mice through the Microinjection of Oocytes. J. Vis. Exp. (124), e55765, doi:10.3791/55765 (2017).
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