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Co-localization of Cell Lineage Markers and the Tomato Signal
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Biologie du développement
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Journal JoVE Biologie du développement
Co-localization of Cell Lineage Markers and the Tomato Signal

Co-localization of Cell Lineage Markers and the Tomato Signal

11,942 Views

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10:56 min

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December 28, 2016

DOI:

10.3791/54982-v

DOI:
10.3791/54982-v

•

10:56 min
December 28, 2016

•

1 Views
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1Department of Biomedical Sciences,Texas A&M University College of Dentistry

Summary

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We developed two sets of tracing combinations in Rosa26tdtomato (ubiquitously expressed in all cells)/Cre (specifically expressed in chondrocytes) mice: one with 2.3Col1a1-GFP (specific to osteoblasts) and one with immunofluorescence (specific to bone cells). The data demonstrate the direct transformation of chondrocytes into bone cells.

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Cite this Article

Jing, Y., Hinton, R. J., Chan, K. S., Feng, J. Q. Co-localization of Cell Lineage Markers and the Tomato Signal. J. Vis. Exp. (118), e54982, doi:10.3791/54982 (2016).

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