ジャーナル
/
発生生物学
/
Co-localization of Cell Lineage Markers and the Tomato Signal
JoVE Journal
発生生物学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 発生生物学
Co-localization of Cell Lineage Markers and the Tomato Signal

Co-localization of Cell Lineage Markers and the Tomato Signal

11,942 Views

•

10:56 min

•

December 28, 2016

DOI:

10.3791/54982-v

DOI:
10.3791/54982-v

•

10:56 min
December 28, 2016

•

1 Views
, , ,
1生物医科学科,Texas A&M University College of Dentistry

概要

Automatically generated

We developed two sets of tracing combinations in Rosa26tdtomato (ubiquitously expressed in all cells)/Cre (specifically expressed in chondrocytes) mice: one with 2.3Col1a1-GFP (specific to osteoblasts) and one with immunofluorescence (specific to bone cells). The data demonstrate the direct transformation of chondrocytes into bone cells.

Explore More Videos

Cell Lineage TracingImmunofluorescenceEndochondrogenesisCell FateChondrocytesOsteogenic CellsCell TransformationTumorFrozen SectioningHypertrophic ChondrocytesTamoxifenCre SystemFixationAnesthesiaPeritoneumAbdomenDissection

Read Article

Cite this Article

Jing, Y., Hinton, R. J., Chan, K. S., Feng, J. Q. Co-localization of Cell Lineage Markers and the Tomato Signal. J. Vis. Exp. (118), e54982, doi:10.3791/54982 (2016).

Download .ris file

Copy

Share Video

.

Copy