Journal
/
Entwicklungsbiologie
/
Co-localization of Cell Lineage Markers and the Tomato Signal
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
Co-localization of Cell Lineage Markers and the Tomato Signal

Co-localization of Cell Lineage Markers and the Tomato Signal

11,917 Views

•

10:56 min

•

December 28, 2016

DOI:

10.3791/54982-v

DOI:
10.3791/54982-v

•

10:56 min
December 28, 2016

•

1 Views
, , ,
1Department of Biomedical Sciences,Texas A&M University College of Dentistry

Summary

Automatically generated

We developed two sets of tracing combinations in Rosa26tdtomato (ubiquitously expressed in all cells)/Cre (specifically expressed in chondrocytes) mice: one with 2.3Col1a1-GFP (specific to osteoblasts) and one with immunofluorescence (specific to bone cells). The data demonstrate the direct transformation of chondrocytes into bone cells.

Explore More Videos

Cell Lineage TracingImmunofluorescenceEndochondrogenesisCell FateChondrocytesOsteogenic CellsCell TransformationTumorFrozen SectioningHypertrophic ChondrocytesTamoxifenCre SystemFixationAnesthesiaPeritoneumAbdomenDissection

Read Article

Cite this Article

Jing, Y., Hinton, R. J., Chan, K. S., Feng, J. Q. Co-localization of Cell Lineage Markers and the Tomato Signal. J. Vis. Exp. (118), e54982, doi:10.3791/54982 (2016).

Download .ris file

Copy

Share Video

.

Copy