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Developmental Biology

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JoVE Journal Developmental Biology
Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors
 

Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors

Article DOI: 10.3791/53421-v • 10:02 min • December 14th, 2015
December 14th, 2015 •
1, 1, 2
1MRC Centre of Developmental Neurobiology, King's College London, 2School of Biological and Chemical Sciences, Queen Mary, University of London

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The cerebellar external granule layer is the site of the largest transit amplification in the developing brain. Here, we present a protocol to target genetic modification to this layer at the peak of proliferation using ex vivo electroporation and culture of cerebellar slices from embryonic Day 14 chick embryos.

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Ex Vivo Culture Chick Cerebellar Slices Spatially Targeted Electroporation Granule Cell Precursors Cerebellar External Granule Layer (EGL) Neuronal Proliferation Differentiation Evolutionary Modifications Cerebellar Size Expansion Amniotes Evo-devo Studies Vertebrate Brain Cerebellar Granule Progenitors Medulloblastoma Paediatric Neuronal Tumour Internal Granular Layer Mature Mammalian Brain Chick Embryos Genetic Modification Ex Vivo Electroporation
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