/
/
Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon
9,445 Views
•
06:02 min
•
September 13, 2019
DOI:
Summary
Automatically generated
Presented here is an electroporation method for plasmid DNA delivery and ependymoglial cell labeling in the adult zebrafish telencephalon. This protocol is a quick and efficient method to visualize and trace individual ependymoglial cells and opens up new possibilities to apply electroporation to a broad range of genetic manipulations.
Explore More Videos
ElectroporationPlasmid DNAIn Vivo DeliveryZebrafish TelencephalonEpendymoglial CellsCell LabelingCell-autonomous ManipulationCell BiologyCell DelaminationEpithelial HomeostasisCell Division SymmetryGlass CapillaryMicroinjectionAnesthesiaStereomicroscopeMicroknifeTelencephalic VentricleUltrasound GelElectroporation
Read Article
Cite this Article
Durovic, T., Ninkovic, J. Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon. J. Vis. Exp. (151), e60066, doi:10.3791/60066 (2019).
Copy