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JoVE Journal Developmental Biology
鸡小脑切片的体外培养和空间上有针对性的颗粒细胞前体电穿孔
 
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鸡小脑切片的体外培养和空间上有针对性的颗粒细胞前体电穿孔

Article DOI: 10.3791/53421-v • 10:02 min • December 14th, 2015
December 14th, 2015 •
1, 1, 2
1MRC Centre of Developmental Neurobiology, King's College London, 2School of Biological and Chemical Sciences, Queen Mary, University of London

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小脑外部颗粒层是最大的过境扩增在显影脑的部位。在这里，我们提出了一个协议，以目标基因改造这个层扩散采用体外电击和小脑切片文化从胚胎第14天鸡胚的高峰。

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发育生物学，第106，小脑，发展，小鸡，外部颗粒层，切片培养，颗粒细胞，浦肯野细胞，电穿孔。
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