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JoVE Journal Developmental Biology
CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer
 

CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer

Article DOI: 10.3791/57311-v • 09:39 min • June 9th, 2018
June 9th, 2018 •
*1, *2, 2, 3,4,5, 1, 3,4
1Division of Molecular Neurogenetics, German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, 2Division of Molecular Genetics, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 3Hopp-Children's Cancer Center at the NCT Heidelberg (KiTZ), 4Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 5Department of Pediatric Hematology and Oncology, Heidelberg University Hospital
* These authors contributed equally

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résumé
Automatic Translation

Conventional loss-of-function studies of genes using knockout animals have often been costly and time-consuming. Electroporation-based CRISPR-mediated somatic mutagenesis is a powerful tool to understand gene functions in vivo. Here, we report a method to analyze knockout phenotypes in proliferating cells of the cerebellum.

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Keywords: CRISPR Loss Of Function Analysis Cerebellar Granule Cells In Utero Electroporation Gene Transfer Developmental Neuroscience Neuro-oncology Cerebellar Development Brain Tumor Formation Immunohistochemistry Knock-out Mouse Plasmid Fast Green
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