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Isolamento ed espansione della cellule staminali neurali del mouse utilizzando il saggio neurosfere
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Neurosciences
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Journal JoVE Neurosciences
Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
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Isolamento ed espansione della cellule staminali neurali del mouse utilizzando il saggio neurosfere

26,514 Views

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13:21 min

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November 20, 2010

DOI:

10.3791/2393-v

DOI:
10.3791/2393-v

•

13:21 min
November 20, 2010

•

12 Views
, , ,
1 Department of Anatomical Sciences,Shiraz University of Medical Sciences, Shiraz, Iran , 2Department of Neurosurgery,University of Florida

Tags

IsolationExpansionAdult MouseNeural Stem CellsNeurosphere AssayPutativeMurine BrainReynolds And WeissChemically Defined Serum-free Culture SystemGrowth Factor Responsive Precursor CellsEpidermal Growth FactorBasic Fibroblastic Growth FactorColoniesUndifferentiated CellsNeurospheresSubculturedPool Of Neural Stem CellsDifferentiateThree Major Cell TypesCNSNeuronsAstrocytesOligodendrocytesInvaluable ToolIn Vitro StudiesTherapeutic PurposesVideo DemonstrationPeriventricular RegionReproducible Neurosphere Cultures

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Azari, H., Rahman, M., Sharififar, S., Reynolds, B. A. Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay. J. Vis. Exp. (45), e2393, doi:10.3791/2393 (2010).

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