/
/
חקירת שיתוף פעולה מיקרוביאלי באמצעות ניתוח ספקטרומטריית מסות הדמיה של מושבות חיידקים הגדלות על אגר וברקמות במהלך זיהום
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Capítulos
- 00:05Introduction
- 00:58Ambient, Vacuum, and Heat-Mediated Drying of Clostridioides difficile and Enterococcus faecalis Bacterial Macrocolonies
- 02:33MALDI Matrix Application via Robotic Spraying
- 03:12Preparing Bacterial Macrocolonies for MALDI Imaging Mass Spectrometry Data Acquisition
- 04:37Imaging Mass Spectrometry Data Analysis and Compound Identification
- 05:57Cryosectioning of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues
- 07:45Preparation of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues for Matrix Application and MALDI Imaging Mass Spectrometry
- 08:08Results: Imaging Mass Spectrometry Analysis of Bacterial Macrocolonies and Infected Murine Cecal Tissues
- 09:09Conclusion
