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Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
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Biochemie
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JoVE Journal Biochemie
Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

1,654 Views

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09:49 min

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November 18, 2022

DOI:

10.3791/64200-v

DOI:
10.3791/64200-v

•

09:49 min
November 18, 2022

•

5 Views
, , , ,
1Department of Chemistry,University of Florida, 2Division of Protective Immunity,Children’s Hospital of Philadelphia, 3Department of Pathology and Laboratory Medicine, Perelman School of Medicine,University of Pennsylvania

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A novel sample preparation method is demonstrated for the analysis of agar-based, bacterial macrocolonies via matrix-assisted laser desorption/ionization imaging mass spectrometry.

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Microbial CooperationImaging Mass SpectrometryBacterial ColoniesAgarMALDI MatrixSample PreparationVacuum DryingSpatial MetabolismMetabolitesMicrobial Pathogens

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Specker, J. T., Smith, A. B., Keenan, O., Zackular, J. P., Prentice, B. M. Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection. J. Vis. Exp. (189), e64200, doi:10.3791/64200 (2022).

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