A novel sample preparation method is demonstrated for the analysis of agar-based, bacterial macrocolonies via matrix-assisted laser desorption/ionization imaging mass spectrometry.
Specker, J. T., Smith, A. B., Keenan, O., Zackular, J. P., Prentice, B. M. Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection. J. Vis. Exp. (189), e64200, doi:10.3791/64200 (2022).