Journal
/
Bioquímica
/
Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

DOI:
10.3791/64200-v

•

09:49 min

•

November 18, 2022

•

, , , ,
1Department of Chemistry,University of Florida, 2Division of Protective Immunity,Children’s Hospital of Philadelphia, 3Department of Pathology and Laboratory Medicine, Perelman School of Medicine,University of Pennsylvania

Capítulos

  • 00:05Introduction
  • 00:58Ambient, Vacuum, and Heat-Mediated Drying of Clostridioides difficile and Enterococcus faecalis Bacterial Macrocolonies
  • 02:33MALDI Matrix Application via Robotic Spraying
  • 03:12Preparing Bacterial Macrocolonies for MALDI Imaging Mass Spectrometry Data Acquisition
  • 04:37Imaging Mass Spectrometry Data Analysis and Compound Identification
  • 05:57Cryosectioning of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues
  • 07:45Preparation of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues for Matrix Application and MALDI Imaging Mass Spectrometry
  • 08:08Results: Imaging Mass Spectrometry Analysis of Bacterial Macrocolonies and Infected Murine Cecal Tissues
  • 09:09Conclusion

Summary

Traducción Automática

Traducción Automática

A novel sample preparation method is demonstrated for the analysis of agar-based, bacterial macrocolonies via matrix-assisted laser desorption/ionization imaging mass spectrometry.

Tags

Microbial CooperationImaging Mass SpectrometryBacterial ColoniesAgarMALDI MatrixSample PreparationVacuum DryingSpatial MetabolismMetabolitesMicrobial Pathogens

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Read Article