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基于斑马鱼和青鳉鱼的成年视锥刺伤损伤模型的再生能力比较分析

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Biología del desarrollo

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JoVE Revista Biología del desarrollo

Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity

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基于斑马鱼和青鳉鱼的成年视锥刺伤损伤模型的再生能力比较分析

DOI:

10.3791/63166-v

•

06:12 min

•

February 10, 2022

•

Yuki Shimizu², Takashi Kawasaki

¹Biomedical Research Institute,National Institute of Advanced Industrial Science and Technology, ²DBT-AIST International Laboratory for Advanced Biomedicine,National Institute of Advanced Industrial Science and Technology

Capítulos

00:04Introduction
00:55Stab Wound Injury in the Adult Optic Tectum
01:34Brain Dissection
03:01Preparation of Frozen Sections
04:44Results: Fluorescent Immunostaining Against RGC Proliferation and the Generation of Newborn Neurons After Tectum Injury
05:28Conclusion

Summary

Traducción Automática

描述了成年斑马鱼的机械脑损伤模型，以研究调节其高再生能力的分子机制。该方法解释了在多种小鱼的视顶上造成刺伤，以使用荧光免疫染色评估再生反应。

Tags

Stab Wound Injury Optic Tectum Zebrafish Medaka Regenerative Capacity Brain Injury RGC Proliferation Differentiation Needle-mediated Injury Central Nervous System Regenerative Therapy PBS Perfusion Paraformaldehyde Fixation

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