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使用全载免疫作用化学和三维重建对咽弓动脉的可视化与分析
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Biología del desarrollo
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JoVE Revista Biología del desarrollo
Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction
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使用全载免疫作用化学和三维重建对咽弓动脉的可视化与分析

DOI:
10.3791/60797-v

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10:02 min

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March 31, 2020

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1Department of Cell Biology and Molecular Medicine, New Jersey Medical School,Rutgers Biomedical and Health Sciences, 2Multidisciplinary PhD Program in Biomedical Sciences: Cell Biology, Neuroscience and Physiology Track, New Jersey Medical School,Rutgers Biomedical and Health Sciences

Capítulos

  • 00:04Introduction
  • 00:55Embryo Staining and Embedding
  • 03:03Methanol Dehydration and Tissue Clearing
  • 04:04Embryo Mounting
  • 04:40Third Pharyngeal Arch (PA) Surfacing
  • 05:57Surfaced Structure Masking
  • 07:01Endothelial Cell Quantification
  • 08:19Results: Representative PA and PAA Surfacing and EC Quantification
  • 09:28Conclusion

Summary

Traducción Automática

Traducción Automática

在这里，我们描述了一种协议，使用全孔免疫荧光、组织清除、共聚焦显微镜和3D重建来可视化和分析小鼠胚胎的咽弓动脉3、4和6。

Tags

VisualizationAnalysisPharyngeal Arch ArteriesWhole-mount Immunohistochemistry3D ReconstructionEndothelial Cell QuantificationAortic Arch ArteriesCongenital Heart DiseaseVessel FormationRemodelingLight-sheet MicroscopyMouse EmbryoFixationPermeabilizationBlocking BufferPrimary AntibodySecondary AntibodyPlastic Paraffin Molds

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