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Visualisation et analyse des artères pharyngées à l’aide de l’immunohistochimie à monture entière et de la reconstruction 3D
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JoVE Revista Biología del desarrollo
Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction
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Visualisation et analyse des artères pharyngées à l’aide de l’immunohistochimie à monture entière et de la reconstruction 3D

9,285 Views

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10:02 min

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March 31, 2020

DOI:

10.3791/60797-v

DOI:
10.3791/60797-v

•

10:02 min
March 31, 2020

•

1 Views
,
1Department of Cell Biology and Molecular Medicine, New Jersey Medical School,Rutgers Biomedical and Health Sciences, 2Multidisciplinary PhD Program in Biomedical Sciences: Cell Biology, Neuroscience and Physiology Track, New Jersey Medical School,Rutgers Biomedical and Health Sciences

Summary

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Ici, nous décrivons un protocole pour visualiser et analyser les artères pharyngées d’arc 3, 4, et 6 des embryons de souris utilisant l’immunofluorescence entière de montage, le dégagement de tissu, la microscopie confocale, et la reconstruction 3D.

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Cite this Article

Ramirez, A., Astrof, S. Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction. J. Vis. Exp. (157), e60797, doi:10.3791/60797 (2020).

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