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Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction

Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction

9,300 Views

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10:02 min

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March 31, 2020

DOI:

10.3791/60797-v

DOI:
10.3791/60797-v

•

10:02 min
March 31, 2020

•

1 Views
,
1Department of Cell Biology and Molecular Medicine, New Jersey Medical School,Rutgers Biomedical and Health Sciences, 2Multidisciplinary PhD Program in Biomedical Sciences: Cell Biology, Neuroscience and Physiology Track, New Jersey Medical School,Rutgers Biomedical and Health Sciences

Summary

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Here, we describe a protocol to visualize and analyze the pharyngeal arch arteries 3, 4, and 6 of mouse embryos using whole-mount immunofluorescence, tissue clearing, confocal microscopy, and 3D reconstruction.

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Ramirez, A., Astrof, S. Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction. J. Vis. Exp. (157), e60797, doi:10.3791/60797 (2020).

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