Journal
/
Biología del desarrollo
/
Visualisierung und Analyse von Pharyngeal Arch Arterien mittels ganzer Immunhistochemie und 3D-Rekonstruktion
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction
Please note that all translations are automatically generated. Click here for the English version.

Visualisierung und Analyse von Pharyngeal Arch Arterien mittels ganzer Immunhistochemie und 3D-Rekonstruktion

9,285 Views

•

10:02 min

•

March 31, 2020

DOI:

10.3791/60797-v

DOI:
10.3791/60797-v

•

10:02 min
March 31, 2020

•

1 Views
,
1Department of Cell Biology and Molecular Medicine, New Jersey Medical School,Rutgers Biomedical and Health Sciences, 2Multidisciplinary PhD Program in Biomedical Sciences: Cell Biology, Neuroscience and Physiology Track, New Jersey Medical School,Rutgers Biomedical and Health Sciences

Summary

Automatically generated

Hier beschreiben wir ein Protokoll zur Visualisierung und Analyse der Rachenbogenarterien 3, 4 und 6 von Mausembryonen mit ganzer Immunfluoreszenz, Gewebeklärung, konfokaler Mikroskopie und 3D-Rekonstruktion.

Explore More Videos

VisualizationAnalysisPharyngeal Arch ArteriesWhole-mount Immunohistochemistry3D ReconstructionEndothelial Cell QuantificationAortic Arch ArteriesCongenital Heart DiseaseVessel FormationRemodelingLight-sheet MicroscopyMouse EmbryoFixationPermeabilizationBlocking BufferPrimary AntibodySecondary AntibodyPlastic Paraffin Molds

Read Article

Cite this Article

Ramirez, A., Astrof, S. Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction. J. Vis. Exp. (157), e60797, doi:10.3791/60797 (2020).

Download .ris file

Copy

Share Video

.

Copy