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Visualisatie en analyse van faryngeale boogslagaders met behulp van Whole-mount Immunohistochemie en 3D Reconstructie
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Biología del desarrollo
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JoVE Revista Biología del desarrollo
Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction
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Visualisatie en analyse van faryngeale boogslagaders met behulp van Whole-mount Immunohistochemie en 3D Reconstructie

9,285 Views

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10:02 min

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March 31, 2020

DOI:

10.3791/60797-v

DOI:
10.3791/60797-v

•

10:02 min
March 31, 2020

•

1 Views
,
1Department of Cell Biology and Molecular Medicine, New Jersey Medical School,Rutgers Biomedical and Health Sciences, 2Multidisciplinary PhD Program in Biomedical Sciences: Cell Biology, Neuroscience and Physiology Track, New Jersey Medical School,Rutgers Biomedical and Health Sciences

Summary

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Hier beschrijven we een protocol om de faryngeale boogslagaders 3, 4 en 6 muisembryo's te visualiseren en te analyseren met behulp van immunofluorescentie, weefselclearing, confocale microscopie en 3D-reconstructie.

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Ramirez, A., Astrof, S. Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction. J. Vis. Exp. (157), e60797, doi:10.3791/60797 (2020).

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