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Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity
Capítulos
- 00:05Introduction
- 01:33Cell Isolation
- 02:57Cardiomyocyte Culture
- 04:17Patch-Clamp Experiment
- 05:44Carbon Fiber Preparation
- 07:40Cardiomyocyte Contraction Force Recording
- 10:23Results: Representative Patch-Clamp and Carbon Fiber Recordings
- 11:44Conclusion
We present a protocol for evaluating the electromechanical effects of GtACR1 activation in rabbit cardiomyocytes. We provide detailed information on cell isolation, culturing and adenoviral transduction, and on functional experiments with the patch-clamp and carbon-fiber techniques.
