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Elektromechanische beoordeling van optogenetisch gemoduleerde cardiomyocyteactiviteit
JoVE Revista
Medicina
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JoVE Revista Medicina
Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity
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Elektromechanische beoordeling van optogenetisch gemoduleerde cardiomyocyteactiviteit

7,669 Views

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12:52 min

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March 05, 2020

DOI:

10.3791/60490-v

DOI:
10.3791/60490-v

•

12:52 min
March 05, 2020

•

4 Views
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1Institute for Experimental Cardiovascular Medicine, University Heart Center Freiburg-Bad Krozingen,Medical Center-University of Freiburg, 2Faculty of Medicine,University of Freiburg, 3Faculty of Biology,University of Freiburg

Summary

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We presenteren een protocol voor de evaluatie van de elektromechanische effecten van GtACR1 activering bij konijnencardiomyocyten. We geven gedetailleerde informatie over celisolatie, kweek- en adenovirale transductie, en over functionele experimenten met de patch-clamp en koolstofvezel technieken.

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Cite this Article

Kopton, R. A., Buchmann, C., Moss, R., Kohl, P., Peyronnet, R., Schneider-Warme, F. Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity. J. Vis. Exp. (157), e60490, doi:10.3791/60490 (2020).

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