Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity

Ramona  A. Kopton; Cinthia Buchmann; Robin Moss; Peter Kohl; R&#233;mi Peyronnet; Franziska Schneider-Warme

doi:10.3791/60490

ジャーナル

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医学

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Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity

JoVE Journal
医学

This content is Free Access.

JoVE Journal 医学

Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity

7,663 Views

•

12:52 min

•

March 05, 2020

DOI:

10.3791/60490-v

DOI:

10.3791/60490-v

•

12:52 min

March 05, 2020

•

4 Views

Ramona A. Kopton^1,2,3, Cinthia Buchmann^1,2, Robin Moss^1,2, Peter Kohl^1,2, Rémi Peyronnet^1,2, Franziska Schneider-Warme^1,2

¹Institute for Experimental Cardiovascular Medicine, University Heart Center Freiburg-Bad Krozingen,Medical Center-University of Freiburg, ²Faculty of Medicine,University of Freiburg, ³Faculty of Biology,University of Freiburg

概要

We present a protocol for evaluating the electromechanical effects of GtACR1 activation in rabbit cardiomyocytes. We provide detailed information on cell isolation, culturing and adenoviral transduction, and on functional experiments with the patch-clamp and carbon-fiber techniques.

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Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity

概要

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