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Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity

Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity

DOI:

12:52 min

March 05, 2020

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Capítulos

  • 00:05Introduction
  • 01:33Cell Isolation
  • 02:57Cardiomyocyte Culture
  • 04:17Patch-Clamp Experiment
  • 05:44Carbon Fiber Preparation
  • 07:40Cardiomyocyte Contraction Force Recording
  • 10:23Results: Representative Patch-Clamp and Carbon Fiber Recordings
  • 11:44Conclusion

Summary

Tadução automática

We present a protocol for evaluating the electromechanical effects of GtACR1 activation in rabbit cardiomyocytes. We provide detailed information on cell isolation, culturing and adenoviral transduction, and on functional experiments with the patch-clamp and carbon-fiber techniques.

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