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在彗星分析中使用冷冻组织评估DNA损伤
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JoVE Revista Genética
Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
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在彗星分析中使用冷冻组织评估DNA损伤

9,035 Views

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11:30 min

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March 24, 2020

DOI:

10.3791/59955-v

DOI:
10.3791/59955-v

•

11:30 min
March 24, 2020

•

4 Views
, , ,
1Toxicology Program, ILS, Inc., 2Division of the National Toxicology Program,National Institute of Environmental Health Sciences

Summary

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该协议描述了在尸体解剖时准备高质量冷冻组织样本以评估DNA损伤的几个程序：1） 切碎组织，2） 从胃肠道刮掉上皮细胞，3） 块状组织样品，需要使用组织切碎装置进行均质化。

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Hobbs, C. A., Recio, L., Winters, J., Witt, K. L. Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage. J. Vis. Exp. (157), e59955, doi:10.3791/59955 (2020).

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