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简化的3D 小脑鉴别协议, 可选2D 修改
JoVE Revista
Biología del desarrollo
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JoVE Revista Biología del desarrollo
Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification
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简化的3D 小脑鉴别协议, 可选2D 修改

DOI:
10.3791/56888-v

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10:15 min

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December 09, 2017

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1Department of Pediatrics/Child Neurology, Amsterdam Neuroscience,VU University Medical Center, 2Department of Complex Trait Genetics, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience,Vrije Universiteit Amsterdam

Capítulos

  • 00:05Título
  • 00:443D “Organoid” Differentiation
  • 04:32Alternative 2D Differentiation Culture
  • 08:28Results: 3D Products Exhibit Markers and Structures of Early Neuroepithelium, and 2D Products Exhibit Cerebellar Markers and Functional Neuronal Activity
  • 09:48Conclusion

Summary

Traducción Automática

Traducción Automática

我们描述了一个简化的 hPSCs 3D 差异化协议, 使用定义的培养基和减少生长因子, 能够产生早期的上皮结构和积极的小脑相关标记的细胞集料, 以及一个可选2D. 将细胞分化为单层, 产生功能性神经元。

Tags

Cerebellar DifferentiationNeural Differentiation3D Organoids2D MonolayerHuman Pluripotent Stem CellsFGF2ROCK InhibitorStreamlined ProtocolCost-effectiveDisease Modeling

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