08:28Results: 3D Products Exhibit Markers and Structures of Early Neuroepithelium, and 2D Products Exhibit Cerebellar Markers and Functional Neuronal Activity
We describe a simplified 3D differentiation protocol for hPSCs, using defined medium and reduced growth factors, capable of generating cell aggregates with early neuroepithelial structures and positive for cerebellar-associated markers, as well as an optional 2D modification for differentiating cells as a monolayer to generate functional neurons.