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Biologia do Desenvolvimento
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Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification

Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification

7,365 Views

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10:15 min

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December 09, 2017

DOI:

10.3791/56888-v

DOI:
10.3791/56888-v

•

10:15 min
December 09, 2017

•

2 Views
,
1Department of Pediatrics/Child Neurology, Amsterdam Neuroscience,VU University Medical Center, 2Department of Complex Trait Genetics, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience,Vrije Universiteit Amsterdam

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We describe a simplified 3D differentiation protocol for hPSCs, using defined medium and reduced growth factors, capable of generating cell aggregates with early neuroepithelial structures and positive for cerebellar-associated markers, as well as an optional 2D modification for differentiating cells as a monolayer to generate functional neurons.

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Cite this Article

Holmes, D. B., Heine, V. M. Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification. J. Vis. Exp. (130), e56888, doi:10.3791/56888 (2017).

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