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Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification
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Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification
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December 09, 2017
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We describe a simplified 3D differentiation protocol for hPSCs, using defined medium and reduced growth factors, capable of generating cell aggregates with early neuroepithelial structures and positive for cerebellar-associated markers, as well as an optional 2D modification for differentiating cells as a monolayer to generate functional neurons.
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Holmes, D. B., Heine, V. M. Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification. J. Vis. Exp. (130), e56888, doi:10.3791/56888 (2017).
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