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生物素化细胞表面蛋白的纯化<emRigicephalus microplus</em>上皮细胞
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JoVE Revista Bioquímica
Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
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生物素化细胞表面蛋白的纯化<emRigicephalus microplus</em>上皮细胞

DOI:
10.3791/55747-v

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12:03 min

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July 23, 2017

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, ,
1Queensland Alliance for Agriculture & Food Innovation,The University of Queensland, 2Centre for Comparative Genomics,Murdoch University

Capítulos

  • 00:00Título
  • 00:28Dissection of the Gut Epithelium from R. microplus
  • 01:56Epithelial Cell Dissociation
  • 03:36Isolation of Single Epithelial Cells Using a Percoll Gradient
  • 06:47Cell Surface Biotinylation and the Isolation of Biotinylated Surface Proteins
  • 09:44Representative Results
  • 11:17Conclusion

Summary

Traducción Automática

Traducción Automática

一种基于梯度改性密度离心方法被利用来上皮细胞从扇头蜱microplus肠组织隔离。生物素化表面结合蛋白，并通过链霉抗生物素蛋白磁珠纯化，用于下游应用。

Tags

Biotinylated Cell Surface ProteinsRhipicephalus MicroplusEpithelial Gut CellsPercoll GradientCell DisassociationCell PurificationStreptavidin Magnetic Beads

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