Journal
/
生物化学
/
Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
JoVE 杂志
生物化学
Author Produced
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells

9,278 Views

•

12:03 min

•

July 23, 2017

DOI:

10.3791/55747-v

DOI:
10.3791/55747-v

•

12:03 min
July 23, 2017

•

3 Views
, ,
1Queensland Alliance for Agriculture & Food Innovation,The University of Queensland, 2Centre for Comparative Genomics,Murdoch University

Summary

Automatically generated

A modified density centrifugation gradient-based methodology was utilized to isolate epithelial cells from Rhipicephalus microplus gut tissue. Surface-bound proteins were biotinylated and purified through streptavidin magnetic beads for utilization in downstream applications.

Explore More Videos

Biotinylated Cell Surface ProteinsRhipicephalus MicroplusEpithelial Gut CellsPercoll GradientCell DisassociationCell PurificationStreptavidin Magnetic Beads

Read Article

Cite this Article

Karbanowicz, T. P., Lew-Tabor, A., Rodriguez Valle, M. Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells. J. Vis. Exp. (125), e55747, doi:10.3791/55747 (2017).

Download .ris file

Copy

Share Video

.

Copy