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Biochemistry
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JoVE Journal Biochemistry
Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
 

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells

Article DOI: 10.3791/55747-v • 12:03 min • July 23rd, 2017
July 23rd, 2017 •
1, 1,2, 1
1Queensland Alliance for Agriculture & Food Innovation, The University of Queensland, 2Centre for Comparative Genomics, Murdoch University

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A modified density centrifugation gradient-based methodology was utilized to isolate epithelial cells from Rhipicephalus microplus gut tissue. Surface-bound proteins were biotinylated and purified through streptavidin magnetic beads for utilization in downstream applications.

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Keywords: Biotinylated Cell Surface Proteins Rhipicephalus Microplus Epithelial Gut Cells Percoll Gradient Cell Disassociation Cell Purification Streptavidin Magnetic Beads
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