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Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
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Biochimie
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Journal JoVE Biochimie
Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells

9,278 Views

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12:03 min

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July 23, 2017

DOI:

10.3791/55747-v

DOI:
10.3791/55747-v

•

12:03 min
July 23, 2017

•

3 Views
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1Queensland Alliance for Agriculture & Food Innovation,The University of Queensland, 2Centre for Comparative Genomics,Murdoch University

Summary

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A modified density centrifugation gradient-based methodology was utilized to isolate epithelial cells from Rhipicephalus microplus gut tissue. Surface-bound proteins were biotinylated and purified through streptavidin magnetic beads for utilization in downstream applications.

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Biotinylated Cell Surface ProteinsRhipicephalus MicroplusEpithelial Gut CellsPercoll GradientCell DisassociationCell PurificationStreptavidin Magnetic Beads

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Karbanowicz, T. P., Lew-Tabor, A., Rodriguez Valle, M. Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells. J. Vis. Exp. (125), e55747, doi:10.3791/55747 (2017).

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