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תרבות של Murine העוברי metatarsals: מודל פיסיולוגי של Endochondral התאבנות
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Biología del desarrollo
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JoVE Revista Biología del desarrollo
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification
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תרבות של Murine העוברי metatarsals: מודל פיסיולוגי של Endochondral התאבנות

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07:23 min

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December 03, 2016

DOI:

10.3791/54978-v

DOI:
10.3791/54978-v

•

07:23 min
December 03, 2016

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3 Views
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1Developmental Biology, The Roslin Institute and R(D)SVS,The University of Edinburgh

Summary

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We present a protocol to dissect and culture embryonic day 15 (E15) murine metatarsal bones. This highly physiological ex vivo model of endochondral ossification provides conditions closer to the in vivo situation than cells in monolayer or 3D culture and is a vital tool for investigating bone growth and development.

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MurineEmbryonicMetatarsalsEndochondral OssificationEx Vivo ModelPhysiological ModelSkeletal MineralizationDissectionCell-cell InteractionsCell-matrix InteractionsCulture MediumHind LimbMetatarsalTweezersDissecting Microscope

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Houston, D. A., Staines, K. A., MacRae, V. E., Farquharson, C. Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification. J. Vis. Exp. (118), e54978, doi:10.3791/54978 (2016).

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