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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria
 

Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria

Article DOI: 10.3791/54690-v • 09:57 min • December 14th, 2016
December 14th, 2016 •
1, *1, *1, 1, 1, 1, 2, 1, 1
1Departments of Microbiology & Immunology, Weill Cornell Medical College, 2Medicine, Weill Cornell Medical College
* These authors contributed equally

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The charcoal agar resazurin assay (CARA) is a semi-quantitative, medium-throughput method to assess activity of test agents against mycobacteria that are replicating, non-replicating, or both. The CARA permits rapid evaluation of time- and concentration-dependent activity and identifies parameters to pursue by colony forming unit (CFU) assays.

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