/
/
Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
708 Views
•
06:38 min
•
December 08, 2023
DOI:
1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute,NCR Biotech Science Cluster, 2Clinical Microbiology Division,CSIR-Indian Institute of Integrative Medicine, 3ICAR-Research Complex for Eastern Region, 4Public Health Research Institute,Rutgers University
Summary
Automatically generated
This protocol details the enrichment of native mycobacterial extracellular vesicles (mEVs) from axenic cultures of Mycobacterium smegmatis (Msm) and how mCherry (a red fluorescent reporter)-containing recombinant MsmEVs can be designed and enriched. Lastly, it verifies the novel approach with the enrichment of MsmEVs containing the EsxA protein of Mycobacterium tuberculosis.
Read Article
Cite this Article
Jayaswal, P., Ilyas, M., Singh, K., Kumar, S., Sisodiya, L., Jain, S., Mahlawat, R., Sharma, N., Gupta, V., Atmakuri, K. Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria. J. Vis. Exp. (202), e65138, doi:10.3791/65138 (2023).
Copy