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Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
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Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
Capítulos
- 00:00Título
- 00:10Background and Significance
- 02:08Reagents
- 03:37Preparation
- 05:04Staining
- 07:54Shipping
- 09:46Analysis
- 10:51Representative Data
- 13:42Conclusion
In this report, we demonstrate the staining and analysis steps of a phenotyping assay performed on fresh whole blood to enumerate major innate and adaptive leukocyte populations. We emphasize considerations for performing these procedures in the context of a multicenter clinical trial.
Tags
Peripheral Blood Leukocyte PopulationsMulticenter Clinical TrialsWhole Blood Phenotyping AssayCryopreservationImmune Response EvaluationsGranulocytesB CellsDendritic CellsFresh Leukocytes AssayVolunteer CommitmentsTimeframesWorking HoursLaboratory PersonnelMultiple Centers11-color Antibody Staining PanelTrucount TubesPhenotype Enumeration
