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Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
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Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
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April 16, 2021
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A protocol is presented to extract the total lipid content of the cell wall of a wide range of mycobacteria. Moreover, extraction and analytical protocols of the different types of mycolic acids are shown. A thin-layer chromatographic protocol to monitor these mycobacterial compounds is also provided.
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Guallar-Garrido, S., Luquin, M., Julián, E. Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography. J. Vis. Exp. (170), e62368, doi:10.3791/62368 (2021).
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