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בידוד של ההסתננות Leukocytes מעור העכבר באמצעות אנזימתי לעכל והפרדת צבע
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Inmunología y contagio
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Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation
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בידוד של ההסתננות Leukocytes מעור העכבר באמצעות אנזימתי לעכל והפרדת צבע

20,788 Views

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07:11 min

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January 25, 2016

DOI:

10.3791/53638-v

DOI:
10.3791/53638-v

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07:11 min
January 25, 2016

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3 Views
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1Department of Biology,Macalester College, 2Department of Medicine, Division of Rheumatic and Autoimmune Diseases, Center for Immunology,University of Minnesota

Tags

LeukocytesInfiltrationSkinEnzymatic DigestGradient SeparationMouse ModelAtopic DermatitisSkin PathologiesHBSSEDTAHEPESFBSCollagenase DDensity Gradient

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Cite this Article

Benck, C. J., Martinov, T., Fife, B. T., Chatterjea, D. Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation. J. Vis. Exp. (107), e53638, doi:10.3791/53638 (2016).

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