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Immunology and Infection

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JoVE Journal Immunology and Infection
A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis
 

A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis

Article DOI: 10.3791/61609-v • 08:46 min • August 12th, 2020
August 12th, 2020 •
*1, *1,2, 1, 1, 1,2
1Department of Microbiology, Immunology, & Cell Biology, West Virginia University School of Medicine, 2Vaccine Development Center at West Virginia University Health Sciences Center
* These authors contributed equally

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Resumen
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Infection of neonatal mice with bioluminescent E. coli O1:K1:H7 results in a septic infection with significant pulmonary inflammation and lung pathology. Here, we describe procedures to model and further study neonatal sepsis using longitudinal intravital imaging in parallel with enumeration of systemic bacterial burdens, inflammatory profiling, and lung histopathology.

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Neonatal Imaging Model Gram-negative Bacterial Sepsis Bacterial Dissemination Burden Tracking Experimental Model Real-time Monitoring Infection Dynamics Bacterial Dissemination Preclinical Testing Interventions For Neonatal Sepsis Host Control Of Bacteria High-dose Litter Group Low-dose Litter Group Biosafety Level Two Cabinet Postnatal Day Three Or Four Weights Of The Pups Inoculation E.coli Lux Inoculum Biosafety Cabinet Loaded Syringes On Ice Injecting The Neonate Needle Bevel Up Skin And Muscle Space 50 Microliters Of Inoculant
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