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Zebrafish의 단세포 운명 매핑
JoVE Revista
Biología
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JoVE Revista Biología
Single Cell Fate Mapping in Zebrafish
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Zebrafish의 단세포 운명 매핑

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07:53 min

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October 05, 2011

DOI:

10.3791/3172-v

DOI:
10.3791/3172-v

•

07:53 min
October 05, 2011

•

3 Views
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1Division of Developmental Biology,Cincinnati Children’s Hospital Medical Center, 2Division of Hematology/Oncology,Cincinnati Children’s Hospital Medical Center

Summary

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사파이어 femtosecond 레이저 발진기 : 메소드는 티에서 두 광자 흡수를 사용하는 형광 단백질 갇힌를 포함하는 단일 세포를 photoactivate에 설명되어 있습니다. 운명의지도 photoactivated 세포는 immunohistochemistry가 사용됩니다. 이 기술은 모든 세포 유형에 적용할 수 있습니다.

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Cite this Article

Kohli, V., Rehn, K., Sumanas, S. Single Cell Fate Mapping in Zebrafish. J. Vis. Exp. (56), e3172, doi:10.3791/3172 (2011).

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