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Single Cell Fate Mapping in de zebravis
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Single Cell Fate Mapping in Zebrafish
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Single Cell Fate Mapping in de zebravis

13,196 Views

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07:53 min

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October 05, 2011

DOI:

10.3791/3172-v

DOI:
10.3791/3172-v

•

07:53 min
October 05, 2011

•

3 Views
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1Division of Developmental Biology,Cincinnati Children’s Hospital Medical Center, 2Division of Hematology/Oncology,Cincinnati Children’s Hospital Medical Center

Summary

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Een methode wordt beschreven om enkele cellen met een gekooide fluorescerend eiwit met behulp van twee-foton absorptie van een Ti photoactivate: Sapphire femtoseconde laser oscillator. Het lot kaart brengen van de gefotoactiveerd cel, wordt immunohistochemie gebruikt. Deze techniek kan worden toegepast op elk celtype.

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Cite this Article

Kohli, V., Rehn, K., Sumanas, S. Single Cell Fate Mapping in Zebrafish. J. Vis. Exp. (56), e3172, doi:10.3791/3172 (2011).

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