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Mappatura dei circuiti neuronali inibitori da fotostimolazione scansione laser
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JoVE Revista Neurociencias
Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation
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Mappatura dei circuiti neuronali inibitori da fotostimolazione scansione laser

17,123 Views

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09:50 min

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October 06, 2011

DOI:

10.3791/3109-v

DOI:
10.3791/3109-v

•

09:50 min
October 06, 2011

•

3 Views
, , ,
1Department of Anatomy and Neurobiology, School of Medicine,University of California, Irvine, 2Department of Biomedical Engineering, School of Engineering,University of California, Irvine

Summary

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Questo documento introduce un approccio di combinare fotostimolazione scansione laser con registrazioni cellula intera in topi transgenici che esprimono GFP in popolazioni limitate neuroni inibitori. La tecnica permette per la mappatura estesa e analisi quantitativa dei locali circuiti sinaptici di specifici neuroni inibitori corticali.

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Cite this Article

Ikrar, T., Olivas, N. D., Shi, Y., Xu, X. Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation. J. Vis. Exp. (56), e3109, doi:10.3791/3109 (2011).

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